Kete Tirohanga RT-PCR kirikaa poaka

Whakaahuatanga Poto:

Ka whakamahia e tenei kete te tikanga RT-PCR rewharewha tuuturu ki te kite i te RNA o te huaketo Swine fever (CSFV) i roto i nga taonga mate kiko penei i te tonsils, nga ngongo lymph me nga taonga mate pukupuku me te wai penei i te kano kano me te toto o te poaka.


Taipitopito Hua

Tohu Hua

Ingoa Hua

Kete Tirohanga RT-PCR (Lyophilized)

Rahi

48whakamatautau/kete, 50whakamatautau/kete

Whakamahinga Whakamahinga

Ka whakamahia e tenei kete te tikanga RT-PCR rewharewha tuuturu ki te kite i te RNA o te huaketo Swine fever (CSFV) i roto i nga taonga mate kiko penei i te tonsils, nga ngongo lymph me nga taonga mate pukupuku me te wai penei i te kano kano me te toto o te poaka.He pai mo te rapunga, te whakamaarama me te tirotiro mate urutaru o te huaketo poaka kirikaa.Ko te kete he PUNA PCR KATOA (Lyophilized), kei roto ko te DNA amplification enzyme, whakamuri transcriptase, parepare tauhohenga, nga tohu tuatahi me nga rangahau e hiahiatia ana mo te rapu PCR rewharewha.

Ihirangi Hua

Waehanga Mōkī whakaritenga Kai whakauru
CSFV PCR Mix 1 ×pounamu (Lyophilized paura)  50Whakamatau dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase
6×0.2ml 8 pai-pupuhi ngongo(Lyophilized) 48Whakamatau
Mana Pai 1*0.2ml ngongo (lyophilized)  10 Nga whakamatautau

Plasmid kei roto CSFV nga kongakonga motuhake

Whakarewa otinga 1.5 ml Cryotube 500uL /
Mana kino 1.5 ml Cryotube 200uL 0.9%NaCl

Rokiroki me te Ora o te Papa

(1) Ka taea te kawe i te kete i te pāmahana rūma.

(2) 18 marama te roa o te noho ki te -20 ℃ me te 12 marama ki te 2 ℃~30 ℃.

(3)Tirohia te tapanga i runga kete mo te ra whakaputa me te ra paunga.

(4)Me penapena te reagent putanga paura lyophilized ki te -20 ℃ i muri i te whakakorenga me te whakatio tukurua - me iti iho i te 4 nga wa.

Nga taputapu

GENECHECKER UF-150, UF-300 taputapu PCR rewharewha o te waa.

Hoahoa Mahi

a) Putanga pounamu:

1

b) 8 putanga pai-pupuri ngongo:

2

Pcr Whakanui

E taunaki anaTautuhinga

Hipanga Porohita Te pāmahana (℃) Te wa Te hongere whiti
1 1 50 8 min  
2 1 95 2min  
3 40 95 5s  
60 10s Kohikohi FAM fluorescence

*Tuhipoka: Ka kohia nga tohu o nga hongere rererangi FAM i te 60 ℃.

Te Whakamaori i nga Hua Whakamatau

Hongere

Te whakamaoritanga o nga hua

FAM Channel

Ct≤35

CSFV Pai

Undet

CSFV kino

35

Hua hihira, whakamatautau ano*

*Mehemea he uara Ct ≤40 te hua whakamatautau tuarua o te hongere FAM me te whakaatu i te anau whakakaha ahua “S”, ka whakamaoritia te hua he pai, ki te kore he kino.


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