中文Pseudorabies huaketo (gB) kete kimi PCR
Ingoa Hua
Pseudorabies huaketo (gB) Kete Rapu PCR (Lyophilized)
Rahi
48whakamatautau/kete, 50whakamatautau/kete
Whakamahinga Whakamahinga
Ka whakamahia e tenei kete te tikanga PCR rewharewha o te waa ki te kite i te RNA o te Pseudorabies huaketo (gB ira) (PRV) i roto i nga taonga mate kiko penei i te tonsils, nga ngongo lymph me nga taonga mate pukupuku me te wai penei i te kano kano me te toto o te poaka.He pai mo te rapunga, te tātari me te tirotiro mate urutaru o te huaketo Pseudorabies (ira gB).Ko te kete he PUNA PCR KATOA (Lyophilized), kei roto ko te enzyme amplification DNA, te parepare tauhohenga, nga tohu tuatahi me nga rangahau e hiahiatia ana mo te rapunga RT-PCR rewharewha.
Ihirangi Hua
| Waehanga | Mōkī | whakaritenga | Kai whakauru |
| PRV PCR Mix | 1 ×pounamu (Lyophilized paura) | 50Whakamatau | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6×0.2ml 8 pai-pupuhi ngongo(Lyophilized) | 48Whakamatau | ||
| Mana Pai | 1*0.2ml ngongo (lyophilized) | 10 Nga whakamatautau | Plasmid, Pseudovirus kei roto PRV(gB) nga kongakonga motuhake |
| Whakarewa otinga | 1.5 ml Cryotube | 500uL | / |
| Mana kino | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Rokiroki me te Ora Papa
(1) Ka taea te kawe i te kete i te pāmahana rūma.
(2) 18 marama te roa o te noho ki te -20 ℃ me te 12 marama ki te 2 ℃~30 ℃.
(3)Tirohia te tapanga kei runga kete mo te ra whakaputa me te ra paunga.
(4)Me penapena te reagent putanga paura lyophilized ki te -20 ℃ i muri i te whakakorenga me te whakatio tukurua - me iti iho i te 4 nga wa.
Nga taputapu
GENECHECKER UF-150, UF-300 taputapu PCR rewharewha o te waa.
Hoahoa Mahi
a) Putanga pounamu:
b) 8 putanga pai-pupuri ngongo:
Pcr Whakanui
Tautuhinga Manakohia
| Hipanga | Porohita | Te pāmahana (℃) | Te wa | Te hongere whiti |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Kohikohi FAM fluorescence |
*Tuhipoka: Ka kohia nga tohu o nga hongere rererangi FAM i te 60 ℃.
Te Whakamaori i nga Hua Whakamatau
| Hongere | Te whakamaoritanga o nga hua |
| FAM Channel | |
| Ct≤35 | PRV(gB) Pai |
| Undet | PRV(gB) Toraro |
| 35 | Whakahounga whakapae, whakamatautau ano* |
*Mēnā he Ct uara ≤40 te hua whakamātautau anō o te hongere FAM me te whakaatu i te ānau whakakaha ahua “S”, ka whakamaoritia te hua he pai, ki te kore he kino.
