Pseudorabies huaketo (gB) kete kitenga PCR

Whakaahuatanga Poto:

Ka whakamahia e te kete nei te tikanga PCR rererangi tuuturu kia kitea te RNA o te Pseudorabies huaketo (ira gB) (PRV) kei roto i nga taonga mate kiko pera i te koromatua, i nga kohinga lymph me nga kiko me nga mate mate waipiro penei i te kano kano me te toto poaka.


Taipitopito Hua

Tohu Hua

Ingoa Hua

Huaketo Pseudorabies (gB) Kete Hoko PCR (Lyophilized)

Rahi

48tautau / kete, 50 whakamatautau / kete

Whakamahi Whakamahia

Ka whakamahia e te kete nei te tikanga PCR rererangi tuuturu kia kitea te RNA o te Pseudorabies huaketo (ira gB) (PRV) kei roto i nga taonga mate kiko pera i te koromatua, i nga kohinga lymph me nga kiko me nga mate mate waipiro penei i te kano kano me te toto poaka. He pai mo te rapunga, te tirotiro me te tirotiro i te mate uruta ki te Pseudorabies virus (ira gB). Ko te kete nei he KOTAHI PCR-KATOA PCR (Lyophilized), kei roto nei i te haakaha te whakanui i te DNA, te parenga tauhohenga, nga waahanga tuatahi me nga tirotirohanga e hiahiatia ana mo te kitenga RT-PCR fluorescent.

Hua o te Hua

Nga Waeata Mōkihi whakamāramatanga Kai
PRV PCR Whakaranu 1 × pounamu (Paura Lyophilized)  50Test dNTPs, MgCl2, Paraimere, Putaa, Taq DNA polymerase
6 × 0.2ml 8 ngongo-ngongo pai(Lyophilized) 48Test
Whakahaere Pai 1 * 0.2ml ngongo (lyophilized)  10 Nga Whakamatau

Plasmid, Pseudovirus ranei kei roto ko nga tohatoha PRV (gB)

Te whakaoti rapanga 1.5 ml Cryotube 500uL /
Whakahaukino 1.5 ml Cryotube 200uL 0.9% NaCl

Rokiroki me te Ora Papa

(1) Ka taea te kawe i te kete i te mahana o te ruuma.

(2) Ko te tau whata 18 marama i -20 ℃ me 12 marama i te 2 ℃ ~ 30 ℃.

(3) Tirohia te tapanga kei runga kete mo te ra whakaputa me te ra paunga.

(4) Ko te reagent putanga paura lyophilized me penapena i te -20 ℃ i muri i te whakakorenga me te toenga tiriti - kia iti iho i te 4 nga wa te waatea.

Taputapu

GENECHECKER UF-150, UF-300 taputapu PCR fluorescence tuuturu.

Whakaaturanga Mahi

a) Putanga Pounamu:

1

b) 8 putanga ngongo ngongo-pai:

2

Pcr Whakarei

Whakatakotoranga kua Whakatauhia

Hipanga

Huringa

Temperature (℃)

Huarere karawhiuwhi

1

1

95

2ini

/

2

40

95

5s

/

60

10s

Kohikohia te fluorescence FAM

* Panui: Ko nga tohu mo nga hongere FAM ka kohia i te 60 ℃.

Te whakamaoritanga i nga Hua Whakamatautau

Channel

Te whakamaoritanga i nga kitenga

Channel FAM

Ct≤35

PRV (gB) Pai

Wetetāuta

PRV (gB) Kino

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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