Pseudorabies huaketo (gB) kete kitenga PCR
Ingoa Hua
Huaketo Pseudorabies (gB) Kete Hoko PCR (Lyophilized)
Rahi
48tautau / kete, 50 whakamatautau / kete
Whakamahi Whakamahia
Ka whakamahia e te kete nei te tikanga PCR rererangi tuuturu kia kitea te RNA o te Pseudorabies huaketo (ira gB) (PRV) kei roto i nga taonga mate kiko pera i te koromatua, i nga kohinga lymph me nga kiko me nga mate mate waipiro penei i te kano kano me te toto poaka. He pai mo te rapunga, te tirotiro me te tirotiro i te mate uruta ki te Pseudorabies virus (ira gB). Ko te kete nei he KOTAHI PCR-KATOA PCR (Lyophilized), kei roto nei i te haakaha te whakanui i te DNA, te parenga tauhohenga, nga waahanga tuatahi me nga tirotirohanga e hiahiatia ana mo te kitenga RT-PCR fluorescent.
Hua o te Hua
| Nga Waeata | Mōkihi | whakamāramatanga | Kai |
| PRV PCR Whakaranu | 1 × pounamu (Paura Lyophilized) | 50Test | dNTPs, MgCl2, Paraimere, Putaa, Taq DNA polymerase |
| 6 × 0.2ml 8 ngongo-ngongo pai(Lyophilized) | 48Test | ||
| Whakahaere Pai | 1 * 0.2ml ngongo (lyophilized) | 10 Nga Whakamatau |
Plasmid, Pseudovirus ranei kei roto ko nga tohatoha PRV (gB) |
| Te whakaoti rapanga | 1.5 ml Cryotube | 500uL | / |
| Whakahaukino | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Rokiroki me te Ora Papa
(1) Ka taea te kawe i te kete i te mahana o te ruuma.
(2) Ko te tau whata 18 marama i -20 ℃ me 12 marama i te 2 ℃ ~ 30 ℃.
(3) Tirohia te tapanga kei runga kete mo te ra whakaputa me te ra paunga.
(4) Ko te reagent putanga paura lyophilized me penapena i te -20 ℃ i muri i te whakakorenga me te toenga tiriti - kia iti iho i te 4 nga wa te waatea.
Taputapu
GENECHECKER UF-150, UF-300 taputapu PCR fluorescence tuuturu.
Whakaaturanga Mahi
a) Putanga Pounamu:
b) 8 putanga ngongo ngongo-pai:
Pcr Whakarei
Whakatakotoranga kua Whakatauhia
|
Hipanga |
Huringa |
Temperature (℃) |
Wā |
Huarere karawhiuwhi |
|
1 |
1 |
95 |
2ini |
/ |
|
2 |
40 |
95 |
5s |
/ |
|
60 |
10s |
Kohikohia te fluorescence FAM |
* Panui: Ko nga tohu mo nga hongere FAM ka kohia i te 60 ℃.
Te whakamaoritanga i nga Hua Whakamatautau
|
Channel |
Te whakamaoritanga i nga kitenga |
|
Channel FAM |
|
|
Ct≤35 |
PRV (gB) Pai |
|
Wetetāuta |
PRV (gB) Kino |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
