E.coli O157: kete H7 PCR kitenga

Whakaahuatanga Poto:

Escherichia coli O157: Ko te H7 (E.coli O157: H7) he huakita karamu-kino na te puninga Enterobacteriaceae, e whakaputa ana i te nui o te whakamate Vero.


Taipitopito Hua

Tohu Hua

Ingoa Hua

E.coli O157: kete H7 PCR kitenga (Lyophilized)

Rahi

48tautau / kete, 50 whakamatautau / kete

Whakamahi Whakamahia

Escherichia coli O157: Ko te H7 (E.coli O157: H7) he huakita karamu-kino na te puninga Enterobacteriaceae, e whakaputa ana i te nui o te whakamate Vero. I te haumanu, ka kitea ohorere ana ka pa te mamae o te puku me te mate o te wai, whai muri i te mate toto hemorrhagic i etahi ra i muri mai, ka mate pea te kirika, kaore he kirika, ka mate i nga keehi tino kino. He pai tenei kete mo te rapu kounga o Escherichia coli O157: H7 i te kai, i nga tauira wai, i te paru, i te ruaki, i te wai whakarei-i te kitakita me etahi atu tauira e whakamahi ana i te kaupapa o te PCR tino tuuturu. Lyophilized), kei roto nei i te haakinakina te whakanui i te DNA, te urupare urupare, nga mea tuatahi me nga tirotiro kia hiahiatia mo te kitenga PCR fluorescent ..

Hua o te Hua

Nga Waeata Mōkihi whakamāramatanga Kai
E.coli O157: H7 PCR Whakaranu 1 × pounamu (Paura Lyophilized)  50Test dNTPs, MgCl2, Paraimere, Take, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 ngongo-ngongo pai(Lyophilized) 48Test
Whakahaere Pai 1 * 0.2ml ngongo (lyophilized)  10 Nga Whakamatau

Plasmid kei roto ko E.coli O157: nga waahanga motuhake H7

Te whakaoti rapanga 1.5 ml Cryotube 500uL /
Whakahaukino 1.5 ml Cryotube 200uL 0.9% NaCl

Rokiroki me te Ora Papa

(1) Ka taea te kawe i te kete i te mahana o te ruuma.

(2) Ko te tau whata 18 marama i -20 ℃ me 12 marama i te 2 ℃ ~ 30 ℃.

(3) Tirohia te tapanga kei runga kete mo te ra whakaputa me te ra paunga.

(4) Ko te reagent putanga paura lyophilized me penapena i te -20 ℃ i muri i te whakakorenga me te toenga tiriti - kia iti iho i te 4 nga wa te waatea.

Taputapu

GENECHECKER UF-150, UF-300 taputapu PCR fluorescence tuuturu.

Whakaaturanga Mahi

a) Putanga Pounamu:

1

b) 8 putanga ngongo ngongo-pai:

2

Pcr Whakarei

Tūtohutia Whakatakoto

Hipanga     Huringa Temperature (℃)   Huarere karawhiuwhi
1 1 95 2ini  
2 40 95 5s  
60 10s Kohikohia te fluorescence FAM

* Panui: Ko te tohu mo te hongere FAM fluorescence ka kohia i te 60 ℃.

Te whakamaoritanga i nga Hua Whakamatautau

Channel

Te whakamaoritanga i nga kitenga

Channel FAM

Ct≤35

E.coli O157: H7 Pai

Wetetāuta

E.coli O157: H7 Kino

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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