Salmonella PCR Kit Rapu

Whakaahuatanga Poto:

Ko Salmonella no te Enterobacteriaceae me te Gram-negative enterobacteria.Ko Salmonella he tukumate noa e kawe ana i te kai me te noho tuatahi mo te paihana kai huakita.


Taipitopito Hua

Tohu Hua

Ingoa Hua

Kete Tirohanga PCR Salmonella (lyophilized)

Rahi

48whakamatautau/kete, 50whakamatautau/kete

Whakamahinga Whakamahinga

Ko Salmonella no te Enterobacteriaceae me te Gram-negative enterobacteria.Ko Salmonella he tukumate noa e kawe ana i te kai me te noho tuatahi mo te paihana kai huakita.Ko nga tohu matua o te paihana kai na Salmonella ko te nausea, te ruaki, te mamae o te puku, te mahunga, te makariri me te korere.Ka whakamahia e tenei kete te maataapono o te PCR rewharewha-a-waa, he pai mo te rapunga tohu o Salmonella i roto i nga kai, nga tauira wai, nga tai, te ruaki, me te wai whakarangatira.

Ihirangi Hua

Waehanga Mōkī whakaritenga Kai whakauru
Whakaranu PCR 1 ×pounamu (Lyophilized paura)  50Whakamatau dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase
6×0.2ml 8 pai-pupuhi ngongo(Lyophilized) 48Whakamatau
Mana Pai 1*0.2ml ngongo (lyophilized)  10 Nga whakamatautau

Plasmid, Pseudovirus ranei kei roto nga waahanga motuhake

Whakarewa otinga 1.5 ml Cryotube 500uL /
Mana kino 1.5 ml Cryotube 100uL 0.9%NaCl

Rokiroki me te Ora o te Papa

(1) Ka taea te kawe i te kete i te pāmahana rūma.

(2) 18 marama te roa o te noho ki te -20 ℃ me te 12 marama ki te 2 ℃~30 ℃.

(3)Tirohia te tapanga i runga kete mo te ra whakaputa me te ra paunga.

(4)Me penapena te reagent putanga paura lyophilized ki te -20 ℃ i muri i te whakakorenga me te whakatio tukurua - me iti iho i te 4 nga wa.

Nga taputapu

GENECHECKER UF-150, UF-300 taputapu PCR rewharewha o te waa.

Hoahoa Mahi

a) Putanga pounamu:

1

b) 8 putanga pai-pupuri ngongo:

2

Pcr Whakanui

Tautuhinga Manakohia

Hipanga

Porohita

Te pāmahana (℃)

Te wa

Te hongere whiti

1

1

95

2min

/

2

40

95

5s

/

60

10s

Kohikohi FAM fluorescence

Te Whakamaori i nga Hua Whakamatau

Hongere

Te whakamaoritanga o nga hua

FAM Channel

Ct≤35

Salmonella Pai

Undet

Salmonella Negative

35

Whakahounga whakapae, whakamatautau ano*

*Mehemea he uara Ct ≤40 te hua whakamatautau tuarua o te hongere FAM me te whakaatu i te anau whakakaha ahua “S”, ka whakamaoritia te hua he pai, ki te kore he kino.


  • Tōmua:
  • Panuku:

  • Hua e Pa ana