中文Kete Tirohanga RT-PCR kirikaa poaka
Ingoa Hua
Kete Tirohanga RT-PCR (Lyophilized)
Rahi
48whakamatautau/kete, 50whakamatautau/kete
Whakamahinga Whakamahinga
Ka whakamahia e tenei kete te tikanga RT-PCR rewharewha tuuturu ki te kite i te RNA o te huaketo Swine fever (CSFV) i roto i nga taonga mate kiko penei i te tonsils, nga ngongo lymph me nga taonga mate pukupuku me te wai penei i te kano kano me te toto o te poaka.He pai mo te rapunga, te whakamaarama me te tirotiro mate urutaru o te huaketo poaka kirikaa.Ko te kete he PUNA PCR KATOA (Lyophilized), kei roto ko te DNA amplification enzyme, whakamuri transcriptase, parepare tauhohenga, nga tohu tuatahi me nga rangahau e hiahiatia ana mo te rapu PCR rewharewha.
Ihirangi Hua
| Waehanga | Mōkī | whakaritenga | Kai whakauru |
| CSFV PCR Mix | 1 ×pounamu (Lyophilized paura) | 50Whakamatau | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6×0.2ml 8 pai-pupuhi ngongo(Lyophilized) | 48Whakamatau | ||
| Mana Pai | 1*0.2ml ngongo (lyophilized) | 10 Nga whakamatautau | Plasmid kei roto CSFV nga kongakonga motuhake |
| Whakarewa otinga | 1.5 ml Cryotube | 500uL | / |
| Mana kino | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Rokiroki me te Ora o te Papa
(1) Ka taea te kawe i te kete i te pāmahana rūma.
(2) 18 marama te roa o te noho ki te -20 ℃ me te 12 marama ki te 2 ℃~30 ℃.
(3)Tirohia te tapanga i runga kete mo te ra whakaputa me te ra paunga.
(4)Me penapena te reagent putanga paura lyophilized ki te -20 ℃ i muri i te whakakorenga me te whakatio tukurua - me iti iho i te 4 nga wa.
Nga taputapu
GENECHECKER UF-150, UF-300 taputapu PCR rewharewha o te waa.
Hoahoa Mahi
a) Putanga pounamu:
b) 8 putanga pai-pupuri ngongo:
Pcr Whakanui
E taunaki anaTautuhinga
| Hipanga | Porohita | Te pāmahana (℃) | Te wa | Te hongere whiti |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Kohikohi FAM fluorescence |
*Tuhipoka: Ka kohia nga tohu o nga hongere rererangi FAM i te 60 ℃.
Te Whakamaori i nga Hua Whakamatau
| Hongere | Te whakamaoritanga o nga hua |
| FAM Channel | |
| Ct≤35 | CSFV Pai |
| Undet | CSFV kino |
| 35 | Hua hihira, whakamatautau ano* |
*Mehemea he uara Ct ≤40 te hua whakamatautau tuarua o te hongere FAM me te whakaatu i te anau whakakaha ahua “S”, ka whakamaoritia te hua he pai, ki te kore he kino.
