中文Shigella PCR Kit Rapu
Ingoa Hua
Kete Tirohanga PCR Shigella (lyophilized)
Rahi
48whakamatautau/kete, 50whakamatautau/kete
Whakamahinga Whakamahinga
Ko te Shigella he momo o te karamu-kino brevis bacilli, no nga tukumate intestinal, me te tukumate tino noa o te mate koriri bacillary tangata.Ka whakamahia e tenei kete te maataapono o te PCR rewharewha tuuturu, e tika ana mo te whakatau i te Shigella i roto i nga kai, nga tauira wai, nga tai, te ruaki, me te wai whakarangatira.
Ihirangi Hua
| Waehanga | Mōkī | whakaritenga | Kai whakauru |
| Whakaranu PCR | 1 ×pounamu (Lyophilized paura) | 50Whakamatau | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6×0.2ml 8 pai-pupuhi ngongo(Lyophilized) | 48Whakamatau | ||
| Mana Pai | 1*0.2ml ngongo (lyophilized) | 10 Nga whakamatautau | Plasmid, Pseudovirus ranei kei roto nga waahanga motuhake |
| Whakarewa otinga | 1.5 ml Cryotube | 500uL | / |
| Mana kino | 1.5 ml Cryotube | 100uL | 0.9%NaCl |
Rokiroki me te Ora o te Papa
(1) Ka taea te kawe i te kete i te pāmahana rūma.
(2) 18 marama te roa o te noho ki te -20 ℃ me te 12 marama ki te 2 ℃~30 ℃.
(3)Tirohia te tapanga i runga kete mo te ra whakaputa me te ra paunga.
(4)Me penapena te reagent putanga paura lyophilized ki te -20 ℃ i muri i te whakakorenga me te whakatio tukurua - me iti iho i te 4 nga wa.
Nga taputapu
GENECHECKER UF-150, UF-300 taputapu PCR rewharewha o te waa.
Hoahoa Mahi
a) Putanga pounamu:
b) 8 putanga pai-pupuri ngongo:
Pcr Whakanui
Tautuhinga Manakohia
| Hipanga | Porohita | Te pāmahana (℃) | Te wa | Te hongere whiti |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Kohikohi FAM fluorescence |
Te Whakamaori i nga Hua Whakamatau
| Hongere | Te whakamaoritanga o nga hua |
| FAM Channel | |
| Ct≤35 | Shigella Pai |
| Undet | Shigella Negative |
| 35 | Whakahounga whakapae, whakamatautau ano* |
*Mehemea he uara Ct ≤40 te hua whakamatautau tuarua o te hongere FAM me te whakaatu i te anau whakakaha ahua “S”, ka whakamaoritia te hua he pai, ki te kore he kino.
