中文E.coli O157:H7 PCR kete rapunga
Ingoa Hua
E.coli O157:H7 PCR kete kimi (Lyophilized)
Rahi
48whakamatautau/kete, 50whakamatautau/kete
Whakamahinga Whakamahinga
Ko te Escherichia coli O157:H7 (E.coli O157:H7) he huakita karamu-kino no te puninga Enterobacteriaceae, e whakaputa ana i te nui o te Vero toxin.I runga i te haumanu, ka puta ohorere mai me te mamae nui o te puku me te mate korere wai, ka whai muri mai i te mate hemorrhagic i etahi ra i muri mai, ka puta te kirikaa, karekau ranei te kirikaa, ka mate te mate.He pai tenei kete mo te rapunga kounga o Escherichia coli O157:H7 i roto i te kai, nga tauira wai, te tai, te ruaki, te wai whakaniko i te huakita me etahi atu tauira ma te whakamahi i te kaupapa o te PCR-waahi.Ko te kete he PUNA PCR KATOA-READY( Lyophilized), kei roto ko te DNA amplification enzyme, te parepare tauhohenga, nga tohu tuatahi me nga rangahau e hiahiatia ana mo te rapu PCR rewharewha.
Ihirangi Hua
| Waehanga | Mōkī | whakaritenga | Kai whakauru |
| E.coli O157:H7 PCR Mix | 1 ×pounamu (Lyophilized paura) | 50Whakamatau | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6×0.2ml 8 pai-pupuhi ngongo(Lyophilized) | 48Whakamatau | ||
| Mana Pai | 1*0.2ml ngongo (lyophilized) | 10 Nga whakamatautau | Plasmid kei roto E.coli O157:H7 nga kongakonga motuhake |
| Whakarewa otinga | 1.5 ml Cryotube | 500uL | / |
| Mana kino | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Rokiroki me te Ora o te Papa
(1) Ka taea te kawe i te kete i te pāmahana rūma.
(2) 18 marama te roa o te noho ki te -20 ℃ me te 12 marama ki te 2 ℃~30 ℃.
(3)Tirohia te tapanga i runga kete mo te ra whakaputa me te ra paunga.
(4)Me penapena te reagent putanga paura lyophilized ki te -20 ℃ i muri i te whakakorenga me te whakatio tukurua - me iti iho i te 4 nga wa.
Nga taputapu
GENECHECKER UF-150, UF-300 taputapu PCR rewharewha o te waa.
Hoahoa Mahi
a) Putanga pounamu:
b) 8 putanga pai-pupuri ngongo:
Pcr Whakanui
E taunaki anaTautuhinga
| Hipanga | Porohita | Te pāmahana (℃) | Te wa | Te hongere whiti |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Kohikohi FAM fluorescence |
*Tuhipoka: Ka kohia te tohu o te hongere fluorescence FAM i te 60 ℃.
Te Whakamaori i nga Hua Whakamatau
| Hongere | Te whakamaoritanga o nga hua |
| FAM Channel | |
| Ct≤35 | E.coli O157:H7 Pai |
| Undet | E.coli O157:H7 Negative |
| 35 | Whakahounga whakapae, whakamatautau ano* |
*Mehemea he uara Ct ≤40 te hua whakamatautau tuarua o te hongere FAM me te whakaatu i te anau whakakaha ahua “S”, ka whakamaoritia te hua he pai, ki te kore he kino.
